human tnbc tmas Search Results


human tnbc tmas #69571112b  (TriStar Technology Group LLC)
90
TriStar Technology Group LLC human tnbc tmas #69571112b
(A) <t>TNBC</t> cells express total and activated forms of EGFR. Whole cell lysate was harvested from six TNBC cell lines and two HER2 positive cell lines. α-Tubulin was used as a loading control. (B) TNBC cells express nEGFR. Cell lines were harvested for nuclear proteins. α-Tubulin, calnexin, and Histone H3 were used as loading and purity controls, respectively. Confocal IF microscopy depicts nEGFR expression. Merged images were magnified to depict nEGFR (Confocal zoom, white arrows). A single Z-Slice image depicts overlap between blue and red signal (white dashed-line boxes). Magnification 600X. EGFR primary antibody specificity was validated with siEGFR and blocking peptides. (C) Immunogold labeling of nEGFR. TNBC cells were fixed and processed for transmission EM. CY, cytoplasm; NE, nuclear envelope; NUC, nucleus; NOS, nucleolus. Images were digitally zoomed to highlight gold particles in the nucleus (black arrows). (D) Human TNBC tumors express nEGFR. IHC staining for EGFR was performed on a total of 74 TNBC patient tumor sections. Representative cases demonstrating nEGFR expression are depicted (black arrows).
Human Tnbc Tmas #69571112b, supplied by TriStar Technology Group LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human tnbc tmas #69571112b/product/TriStar Technology Group LLC
Average 90 stars, based on 1 article reviews
human tnbc tmas #69571112b - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


(A) TNBC cells express total and activated forms of EGFR. Whole cell lysate was harvested from six TNBC cell lines and two HER2 positive cell lines. α-Tubulin was used as a loading control. (B) TNBC cells express nEGFR. Cell lines were harvested for nuclear proteins. α-Tubulin, calnexin, and Histone H3 were used as loading and purity controls, respectively. Confocal IF microscopy depicts nEGFR expression. Merged images were magnified to depict nEGFR (Confocal zoom, white arrows). A single Z-Slice image depicts overlap between blue and red signal (white dashed-line boxes). Magnification 600X. EGFR primary antibody specificity was validated with siEGFR and blocking peptides. (C) Immunogold labeling of nEGFR. TNBC cells were fixed and processed for transmission EM. CY, cytoplasm; NE, nuclear envelope; NUC, nucleus; NOS, nucleolus. Images were digitally zoomed to highlight gold particles in the nucleus (black arrows). (D) Human TNBC tumors express nEGFR. IHC staining for EGFR was performed on a total of 74 TNBC patient tumor sections. Representative cases demonstrating nEGFR expression are depicted (black arrows).

Journal: Molecular cancer therapeutics

Article Title: Nuclear Epidermal Growth Factor Receptor is a Functional Molecular Target in Triple-Negative Breast Cancer

doi: 10.1158/1535-7163.MCT-13-1021

Figure Lengend Snippet: (A) TNBC cells express total and activated forms of EGFR. Whole cell lysate was harvested from six TNBC cell lines and two HER2 positive cell lines. α-Tubulin was used as a loading control. (B) TNBC cells express nEGFR. Cell lines were harvested for nuclear proteins. α-Tubulin, calnexin, and Histone H3 were used as loading and purity controls, respectively. Confocal IF microscopy depicts nEGFR expression. Merged images were magnified to depict nEGFR (Confocal zoom, white arrows). A single Z-Slice image depicts overlap between blue and red signal (white dashed-line boxes). Magnification 600X. EGFR primary antibody specificity was validated with siEGFR and blocking peptides. (C) Immunogold labeling of nEGFR. TNBC cells were fixed and processed for transmission EM. CY, cytoplasm; NE, nuclear envelope; NUC, nucleus; NOS, nucleolus. Images were digitally zoomed to highlight gold particles in the nucleus (black arrows). (D) Human TNBC tumors express nEGFR. IHC staining for EGFR was performed on a total of 74 TNBC patient tumor sections. Representative cases demonstrating nEGFR expression are depicted (black arrows).

Article Snippet: Two human TNBC TMAs (#695711112B and #69572306) were purchased from TriStar Technology Group (Rockville, MD, USA).

Techniques: Control, Microscopy, Expressing, Blocking Assay, Labeling, Transmission Assay, Immunohistochemistry

(A) Constitutively active Src (caSrc) enhances nEGFR translocation in TNBC cell lines. Cells were transfected with caSrc or an empty vector control for 48 hr prior to stimulation with EGF (5 nM, 45 min) to induce nEGFR translocation. Non-nuclear and nuclear proteins were harvested. nEGFR expression was quantitated using ImageJ software (B) A negative regulator of Src, src-like adaptor protein (SLAP), blocks nEGFR translocation in TNBC cell lines. Cells were transfected with SLAP-FLAG or an empty vector control for 48 hr prior to harvesting non-nuclear and nuclear proteins. nEGFR expression was analyzed. (C) SLAP can interact with EGFR and decrease EGFR activation at Tyrosine 1101. Cells were transfected with SLAP-FLAG or an empty vector control for 48 hr prior to harvesting whole cell lysate. 250 ug of cell lysate was immunoprecipitated with an anti-SLAP antibody. The same lysate was subjected to immunoblot analysis for activation of EGFR at Tyrosine 1101. pEGFR-Y1101 activity was quantitated using ImageJ software. Inset 1: EGFR mutated at Tyrosine 1101 is deficient in nuclear localization. Vector, EGFR-WT, and EGFR-Y1101F were transfected into CHOK1 cells for 48 hr prior to stimulation with EGF (5 nM, 45 min). Non-nuclear and nuclear proteins were harvested, and nEGFR expression was analyzed.

Journal: Molecular cancer therapeutics

Article Title: Nuclear Epidermal Growth Factor Receptor is a Functional Molecular Target in Triple-Negative Breast Cancer

doi: 10.1158/1535-7163.MCT-13-1021

Figure Lengend Snippet: (A) Constitutively active Src (caSrc) enhances nEGFR translocation in TNBC cell lines. Cells were transfected with caSrc or an empty vector control for 48 hr prior to stimulation with EGF (5 nM, 45 min) to induce nEGFR translocation. Non-nuclear and nuclear proteins were harvested. nEGFR expression was quantitated using ImageJ software (B) A negative regulator of Src, src-like adaptor protein (SLAP), blocks nEGFR translocation in TNBC cell lines. Cells were transfected with SLAP-FLAG or an empty vector control for 48 hr prior to harvesting non-nuclear and nuclear proteins. nEGFR expression was analyzed. (C) SLAP can interact with EGFR and decrease EGFR activation at Tyrosine 1101. Cells were transfected with SLAP-FLAG or an empty vector control for 48 hr prior to harvesting whole cell lysate. 250 ug of cell lysate was immunoprecipitated with an anti-SLAP antibody. The same lysate was subjected to immunoblot analysis for activation of EGFR at Tyrosine 1101. pEGFR-Y1101 activity was quantitated using ImageJ software. Inset 1: EGFR mutated at Tyrosine 1101 is deficient in nuclear localization. Vector, EGFR-WT, and EGFR-Y1101F were transfected into CHOK1 cells for 48 hr prior to stimulation with EGF (5 nM, 45 min). Non-nuclear and nuclear proteins were harvested, and nEGFR expression was analyzed.

Article Snippet: Two human TNBC TMAs (#695711112B and #69572306) were purchased from TriStar Technology Group (Rockville, MD, USA).

Techniques: Translocation Assay, Transfection, Plasmid Preparation, Control, Expressing, Software, Activation Assay, Immunoprecipitation, Western Blot, Activity Assay